UC San Diego Jobs Site

Column Content Left

#105530 Staff Research Associate 1

Extended Deadline: Wed 4/21/2021

This is a 100% limited appointment for 12 months with the possibility of extension or conversion to career status depending on project needs and funding availability.

UCSD Layoff from Career Appointment: Apply by 08/28/2020 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 09/08/2020. Eligible Special Selection clients should contact their Disability Counselor for assistance.

Job posting will remain open until position is filled.

DESCRIPTION

The Villa lab is at the forefront of structural elucidation of macromolecular assemblies within their native biological context. Our group members are curious, highly motivated, and collaborative individuals. The Staff Research Associate 1 will assist the Principal Investigator in conducting scientific research, performing administrative functions for the group, and ensuring the lab is abiding by Environmental Health & Safety standards. Within the research component, there is room for growth, dependent on the progression of the project.

Under supervision, the Staff Research Associate 1 will perform research including mammalian tissue culture experiments and various imaging experiments, including single cell injections into the nucleus of mammalian cells grown in tissue culture conditions. This work will be vital to an ongoing project coordinated by two postdoctoral fellows. Additional mammalian cell work will also include standard tissue culture cell maintenance, transfection of plasmid DNA, fluorescence imaging, etc. Additional research components include generating samples and reagents suitable for the lab's cryo-tomography workflow (culturing cells on grids and microfabrication of EM grids).

Additional functions include administrative tasks such as ordering reagents, maintaining an inventory, and ensuring that supplies are ordered in a timely manner. Finally, the incumbent will handle tasks related to EH&S, as ensuring the group is compliant with safety requirements is of the utmost importance.

  • Must be able to be flexible with work hours and work occasional overtime during evenings or weekends as required by particular experiments.

QUALIFICATIONS

  • Theoretical and practical knowledge of biology, molecular biology or biochemistry acquired through a BS degree or higher in Biology or related field; OR an equivalent combination of education and laboratory experience.

  • Thorough knowledge and experimental ability to perform procedures used in a molecular biology research laboratory setting such as sterile technique, DNA and RNA preparation, protein purification, PCR amplification, bacterial transformation, Western blotting, cloning, bacterial growth and handling, plasmid preparation.

  • Demonstrated experience working with antibodies and yeast.

  • Proven experience with the operation of laboratory equipment, including centrifuges, ultracentrifuges, balances, and pH meters. Knowledge and skill working with sterile equipment and supplies.

  • Demonstrated experience using fluorescent microscope (deconvolution and confocal).

  • Demonstrated skill with stoichiometric calculations, i.e. molarity, etc.

  • Proven knowledge of specialized scientific terminology and ability to perform research under guidance. Ability to record scientific data in a well-organized laboratory notebook.

  • Thorough knowledge of purchasing and reimbursement procedures. Skill in maintaining purchasing records, steady levels of lab supplies, and training others in purchasing procedures.

  • Proficient experience using MAC computers and associated software, including FilemakerPro, MS Word, and Excel.

  • Demonstrated knowledge of PubMed or library literature search to identify relevant research papers, journals and books

  • Knowledge of Material Data Safety Sheets and hazardous communication and Injury and Illness Prevention programs. Knowledge of laboratory safety precautions, including use of protective items such as goggles, coats, etc., and the ability to train other laboratory members in safety precautions.

  • Proven experience and knowledge of proper handling, storage and disposal of chemicals, radioisotopes, biochemicals, hazardous chemicals, and biohazardous materials, including radioactive materials.

  • Excellent verbal and written communication skills. Ability in using tact, diplomacy, discretion, and flexibility with diverse personalities and situation. Ability to work as part of a research team and interact effectively with collaborating labs and others in scientific community.

  • Ability to embrace and consistently uphold the UC San Diego Principles of Community for a collaborative work environment.

PREFERRED QUALIFICATIONS

  • Experience with CRISPR/Cas9 technology.

  • Experience working with iPSC cells, including generation and differentiation of iPSC cells.

  • Some experience in RNA expression analysis using RNAseq and its data analysis.

SPECIAL CONDITIONS

  • Must be willing to work with radioisotopes (35S, 32P).

The effects of the COVID-19 pandemic have impacted UC San Diego Campus hiring. UCSD Campus will continue to recruit for essential positions and we ask for your understanding and patience with our application process during this unprecedented time. For more information regarding steps we are taking to keep applicants and staff safe during this time, please click here. Additional information about UC San Diego’s response to the COVID-19 pandemic: https://returntolearn.ucsd.edu/ and https://blink.ucsd.edu/HR/services/covid-19/index.html.


The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

UCSD
Payroll Title:
SRA 1
Department:
DIVISION OF BIOLOGICAL SCI.
Hiring Salary Range
$23.34 - $24.88 /hour
Worksite:
Campus
Appointment Type:
Limited Appointment
Appointment Percent:
100%
Union:
RX Contract
Total Openings:
1
Work Schedule:
Days, 8 hrs/day, Mon - Fri